Yes. The Steam categories include single-player. I would still read the difficulty as a co-op shooter that lets you go alone, not as a story game built only for one.
Solo retains the class, weapon-grid, and contract structure without another player available to revive you. A cautious starting suggestion is to learn a low-threat objective and its extraction prompt before pursuing hidden weapons. Paid Time Off is a separate activity, not the ordinary exit button.
Classes still matter alone. Glider moves differently from Bruiser. I am not going to claim a solo-only balance patch, because I did not see one called out in the 2.0.2 notes I scanned.
When solo is the wrong expectation
Some Steam guides talk about squad damage and revive loops. Those routes assume a team. If a build video shows four players melting a boss, do not copy the glass-cannon version on your first solo night.
Ouroboros is a separate roguelike mode inside the game. Solo there is a different loop from the mission board. I keep them apart so nobody thinks the whole game is a run-based roguelike.
FAQ
Is Mycopunk playable solo?
Yes. Single-player is an official Steam category.
Is Mycopunk online only?
Steam lists single-player separately from online co-op, so a squad is optional. Those categories alone do not verify offline startup or account requirements.
Is solo harder?
You lose revives from friends. The store does not publish a separate solo difficulty multiplier.
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